Immunofluorescence Protocol For Paraffin Embedded Sections

Immunohistochemistry protocol for paraffin embedded tissue sections advertisement doi.

Immunofluorescence protocol for paraffin embedded sections.

Tissues can be embedded into paraffin using specialized automated tissue processing systems. The suggested cryostat temperature is between 15 and 23 c. Thaw mount the sections onto gelatin coated histological slides. Cut 5 15 µm thick tissue sections using a rotary microtome.

Section the paraffin embedded tissue block at 5 8 µm thickness on a microtome and float in a 40 c water bath containing distilled water. The section will curl if the specimen is too cold. Do not use this pretreatment with frozen sections or cultured cells that are not paraffin embedded. Immunofluorescence general protocol important.

Mount the sections onto either probeon or probeon plus or gelatin coated histological slides. Ihc protocol video for paraffin embedded tissue sections from cell signaling technology cst cst protocols. Cut 5 15 µm thick tissue sections using a cryostat. If the sections still have traces of wax an additional immersion of 5 minutes in xylene may be employed.

Slides are pre coated with gelatin to enhance adhesion of the. The paraffin tissue block can be stored at room temperature for years. Studies performed as early as the 1970s have shown that if p is a valuable salvage technique in renal pathology when frozen tissue is inadequate e g lacks glomeruli or not available 8 10 14 18 20 21 overall diagnostic results by if p can be obtained in 80 of cases 14 18 20 21 but the diagnostic yield varies depending on 3. Download immunofluorescent staining of paraffin embedded tissue protocol as a pdf deparaffinization and rehydration tip.

Paraffin immunofluorescence as a salvage technique. Immunofluorescence microscopy is a method by which a protein can be visualized inside cells using fluorescent dyes. Before moving to alcohol grades step make sure to completely deparaffinize the sections. Rinse sections in pbs tween 20 for 2x2min.

Antigenic determinants masked by formalin fixation and paraffin embedding often may be exposed by epitope umasking enzymatic digestion or saponin etc. Transfer the sections onto glass slides suitable for immunohistochemistry e g. Embed the tissue in paraffin at 58 c. April 10 2014 katie crosby 1 jessica simendinger 1 christopher grange 1 michelle ferrante 1 trisha bernier 1 claire standen 1.

If it is too warm it will stick to the knife. Embed the tissue in a paraffin block.